Table 1.

Risk Factors Associated with Overall Survival

	Model 1		Model 2
	Hazard Ratio	p value	Hazard Ratio	p value
Age (>60 years)	2.72 (1.51-4.90)	<0.001	2.50 (1.41-4.43)	0.0018
Sex (male)	2.30 (1.27-4.17)	0.0062	2.27 (1.25-4.10)	0.0069
Somatic mutations
“Favorable”	0.27 (0.09-0.78)	0.016	--------	--------
“Unmutated”	0.56 (0.29-1.09)	0.088	--------	--------
“Mixed”	0.23 (0.05-1.05)	0.058	--------	--------
“Combined”	--------	--------	0.48 (0.25-0.91)	0.024
Blood counts at diagnosis
Reticulocytes	0.43 (0.22-0.84)	0.014	0.47 (0.25-0.88)	0.019
Neutrophils	0.67 (0.26-1.72)	0.418	0.67 (0.26-1.75)	0.412
Lymphocytes	1.63 (0.89-2.97)	0.110	1.46 (0.83-2.60)	0.192
Platelets	1.18 (0.57-2.46)	0.657	1.34 (0.65-2.79)	0.429

Risk factors were assessed in a Multivariate Cox Proportional Hazard Model. Hazard ratios and 95% confidence intervals (shown in parenthesis) were derived for each variable. Reference (baseline) groups for categorical variables were: age ≤60 years, sex = female, and “unfavorable” mutations. Blood counts at the time of diagnosis were treated as continuous variables after log10 transformation. P-values for each variable were obtained by likelihood ratio tests. In Model 1, individual gene sets (“favorable”, “unmutated” and “mixed” mutations) each were compared separately to the “unfavorable” gene set as a reference. In Model 2, all other gene sets were combined for comparison to the “unfavorable” gene set. Somatic mutation categories were derived using a PVS algorithm, as described in the text, for favorable (PIGA and BCOR/BCORL1), unfavorable mutations (ASXL1, DNMT3A, TP53, RUNX1, and CSMD1), and “unmutated” cases (none of these mutations or no mutations); thirteen patients had mixed mutations.