Table 1.
Sequences of Primers and Probes Used for Real-Time Quantitative PCR of Bacterial Pathogen Nucleic Acid Detection
| Bacterium Name | Primer Sequence (5ʹ→3ʹ) | Annealing Temperature (°C) |
|---|---|---|
| Pg | F: TTCGCTGTGGAAGCTTGACG R: ACTGACACTGAAGCACGAAG P: FAM- GCGTGGGTATCAAACAGGAT-TAMRA |
60.0 |
| Aa | F: TTACGCACATGTCCGAGCAAG R: AGCCATGGTGTTGATTCAGCGT P: FAM- AATCGTCGCTTGTTGAGGCG-TAMRA |
60.0 |
| Pi | F: AGATATCATGACGAACTCCGATTGC R: CGAGCTTAAGCGTCAGTAACACT P: FAM -CCGTACGCTGCCTTC-TAMRA |
60.0 |
| Tf | F: TGGTCCGTGTCTCAGTACC R: TTGCGGATGGGCATGCGTA P: FAM- CCATTAGGTAGTTGGTGAGG-TAMRA |
60.0 |
| Td | F: CCGAATGTGCTCATTTACATAAAGGT R: GATACCCATCGTTGCCTTGGT P: FAM- ATCTCAGTCCCAATGTGTCC-TAMRA |
60.0 |
| Pa | F: TTGGCACCTTCACCGGAAG R: ATGGAAATGCTGAAATTCGG P: FAM- TTGGAGGAGCAACCCACAGC-TAMRA |
60.0 |
| Total | F: GGTAAGGTTCTTCGCGTTGC R: GATTAGATACCCTGGTAGTCCA P: FAM-AACCACATGCTCCACC-MGB |
55.0 |
Abbreviations: PCR, polymerase chain reaction; F, forward primer; R, reverse primer; P, probe; Pg, Porphyromonas gingivalis; Aa, Actinobacillus actinomycetemcomitans; Pi, Prevotella intermedia; Tf, Tannerella forsythensis; Td, Treponema denticola; Pa, Pseudomonas aeruginosa.