Table 2. Sequence analysis of the V2 and V31 rearrangements on opposite alleles in V2^R/+/V31^+/R T cell hybridomas.

	Vβ		N/P	Potential Dβ	N/P	Jβ		Status
Clone 1	V2	AGCAGCCAAGA	TA	A	T	CCGGGCAG	2.2	Out-of-frame
	V31	GCCTGGAGTCT		AC		CCAACGAAAGA	1.4	In-frame
Clone 23	V2	AGCAGCCAAGA	T	TGG		GAACAG	2.7	In-frame
	V31	The V31 rearrangement was not identified
Clone 54	V2	AGCAGC	CAA	GAC		AACACCGGGCAG	2.2	In-frame
	V31	GCCTGGAGTCT	A	GC	G	TGAACAG	2.7	Out-of-frame
Clone 55	V2	AGCAGC	T	T		CTCCTATGAACAG	2.7	In-frame
	V31	GCCTGGAGTCTC		GGGACAGGGGG	GCGG	GAACAG	2.7	In-frame
Clone 59	V2	AGCAGCCAAGA	TC	GG		AGTCAAAACACCTTG	2.4	In-frame
	V31	GCCT		GGC		CACCTTG	2.4	Out-of-frame
Clone 62	V2	The V2(Dβ)Jβ2 rearrangement was not cloned and sequenced
	V31	V31-to-5′Dβ1 RSS hybrid join, deleting all subsequent Jβ1-Cβ1 and Dβ2/Jβ2-Cβ2 sequences
Clone 90	V2	AGCAGC	TT	CT	C	CTATGAACAG	2.7	In-frame
	V31	GCCTGGAGTC	CCCCT	CTGGGGGG	TGCAGAAA	CGCTGTATTTTGGC	2.3	In-frame
Clone 105	V2	AGCAGCCA	CCTCC	GGGACTGGGGC	TATGA	ACAG	2.7	In-frame
	V31	V31 rearranged to the Vβ coding sequence of an out of frame V29(Dβ)Jβ2.7 rearrangement						Out-of-frame

Sequences are underlined every three nucleotides to orient the reading frame. Dβ nucleotides in bold are from Dβ1, italicized nucleotides are from Dβ2, and for V2 rearrangements, unformatted nucleotides could be from either.