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. 2020 Jun 5;217(9):e20192152. doi: 10.1084/jem.20192152

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© 2020 Ningbo University

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Figure 8. — Transfer of ZIKV-elicited CD8⁺ T cells increases the survival of JEV-infected mice. (A and B) 1-d-old naive C57BL/6 mice were r.o. injected with 2 × 10⁶ CD8⁺ T cells from EV71-VP1–immune mice that had been s.c. immunized with EV71-VP1 (50 µg/mouse/each; twice, 2 wk apart) 28 d earlier (n = 9) or ZIKV-exposed mice injected r.o. with ZIKV (10² PFU) 28 d earlier (n = 9). 2 h later, the neonates were injected s.c. with JEV (12 PFU). Mouse weights (A) and survival (B) were recorded daily for 14 d. Data are presented as the mean ± SD and are pooled from two experiments, each with four or five mice/group. *, P < 0.05; ***, P < 0.001 by two-tailed Mann–Whitney U test (A) or log-rank test (B).