Figure S3.

JEV- and ZIKV-reactive IgG in the serum of mice 6 h after transfer of mouse JEV-immune serum. (A–F) 1-d-old naive C57BL/6 mice were injected s.c. with 3, 15, or 75 µl of mock- or JEV-immune mouse sera (n = 5 for all groups). 6 h later, the mice were sacrificed, and sera were collected. JEV-EDIII– and ZIKV-EDIII–reactive IgG levels were measured by indirect ELISA. Data are presented as the mean ± SEM. **, P < 0.01 by two-tailed Mann–Whitney U test.