Figure S4.
Maternally acquired anti-ZIKV Abs have no effect on the survival of JEV-infected pups born to ZIKV-immune mothers. 6-wk-old female C57BL/6 mice were i.p. injected with 1 mg anti-Ifnar1 mAb (MAR1-5A3) 1 d before r.o. injection with ZIKV (102 FFU). 4 wk later, naive or ZIKV-immune females were mated with 10-wk-old naive male mice. (A and B) 1-d-old C57BL/6 pups were sacrificed, and sera were prepared. ZIKV-EDIII and JEV-EDIII–reactive IgG were measured by indirect ELISA. Mouse anti-His mAb was used as positive control. Data are presented as the mean ± SEM. (C and D) 1-d-old C57BL/6 pups were injected s.c. with JEV (12 PFU). Mouse weights and survival were recorded daily for 14 d. Data are presented as the mean ± SD and are pooled from four experiments totaling 23 infected mice born to 4 naive mothers and 25 infected mice born to 4 ZIKV-immune mothers. *, P < 0.05; ****, P < 0.0001 by two-tailed Mann–Whitney U test.