Figure S2.

Comparative scRNA-seq analysis of human melanoma CD8⁺ TIL subsets. (A) Gene set enrichment analysis showing enrichment of exhaustion, tissue-resident memory, and activation and effector function signatures genes within the ranked gene expression of PD-1^hiCTLA-4^hi compared with PD-1^loCLTA-4^lo CD8⁺ TILs from human melanoma (n = 5). NES, normalized enrichment score. (B) Matched tissue scRNA-seq of CD8⁺ T cells isolated from patient K-411. (C) UMAP plots generated from scRNA-seq and scTCR-seq demonstrating LAYN expression and clone size from K-409 primary tumor. Clones are defined as sets of cells with perfect matches for all called TCR α and β chains from single-cell TCR data. (D) Coxcomb plots showing the 20 most expanded LAYN⁺ and LAYN⁻ clones in K-409 primary tumor. Each pie slice represents a unique CD8⁺ T cell clonotype, and pie slice height is proportional to clone size. FDR, false discovery rate.