Figure 5.

Administration of NS1(D2Y98P) to AG129 mice infected with a sublethal dose of D2Y98P. (A) AG129 mice were challenged s.c. with 10³ PFU D2Y98P. (B–H) At day 2 p.i. (B–F) or day 4 p.i. (E–H), infected mice were i.v. administered 10 mg/kg NS1(D2Y98P) or OVA. (B and E) Systemic levels of sNS1 were measured at various time points p.i. (n = 4–5). (C, D, and F) Vascular leakage was evaluated in various tissues at day 4 (C) or day 6 (D and F) p.i. (n = 4–5). (G and H) Plasma heparan sulfate (n = 4; G) and TNF-α levels (n = 4; H) were measured at day 6 p.i. Data are representative of at least two independent experiments. Data were analyzed by nonparametric Mann–Whitney U test. *, P < 0.05; **, P < 0.01; ns, not significant. Results were expressed as averages ± SD (B–F).