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. 2020 Jun 25;217(9):e20191548. doi: 10.1084/jem.20191548

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© 2020 Lee et al.

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Figure 6. — Passive NS1 immunity in an ADE model using D220-NS1-D2Y98P chimeric virus. (A) D220-NS1-D2Y98P chimeric virus consists of NS1 coding region from D220 strain (blue) in the backbone of D2Y98P strain (red). AG129 mice born to DENV1-immune mothers were passively transferred with NS1 immune serum as described in Fig. 2 A. 1 d after transfer, the mice were challenged s.c. with 10³ PFU of chimeric D220-NS1-D2Y98P. (B and C) Systemic levels of sNS1 (B) and viremia titers (C) were measured at days 2, 4, and 6 p.i. (n = 5). (D and E) Mice (n = 8) were monitored daily upon challenge. Kaplan–Meier survival curve and clinical score are shown. (F) Vascular leakage was assessed in various tissues at day 6 p.i. (n = 5). Data are representative of at least two independent experiments. Data were analyzed by nonparametric Mann–Whitney U test. **, P < 0.01; ns, not significant. Results were expressed as averages ± SD (B and F).