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. 2020 Jun 25;217(9):e20191548. doi: 10.1084/jem.20191548

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© 2020 Lee et al.

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Figure S1. — NS1-specific IgG titers in NS1 immune serum. A129 mice were immunized i.p. three times with 20 µg NS1(16681) or OVA with MPLA and Addavax as adjuvants at wks 2, 6, and 8. (A and B) NS1-specific IgG antibody titers were measured by ELISA using NS1(16681) (A) or NS1(D2Y98P) (B) as coating antigen. Titers were calculated as the reciprocal of the highest serum dilution with absorbance corresponding to 3× the absorbance of blank wells. The dotted line indicates the limit of detection. (C) The percentages of specific IgG subclasses were determined. (D) 5×-diluted NS1 immune serum was coincubated with 20 µg/ml NS1(16681) or NS1(D2Y98P), and the amount of free NS1 was measured by sandwich ELISA.