Skip to main content
. 2020 Jun 10;217(9):e20200164. doi: 10.1084/jem.20200164

Figure S2.

Figure S2.

Association between IL-33 and erythroid progenitors. (A) Frequency of transcript counts for indicated cytokine receptor genes in indicated progenitor populations. Gene for the IL-33 receptor, ST2, is highlighted. Data derived from Haemosphere RNA-sequencing database of BM progenitors. Bars represent mean and SEM of two biological replicates. (B) Volcano plot comparing gene expression between GMP and CFU-E. The IL-33 receptor gene, Il1rl1, is highlighted, along with critical erythroid (Gata1) and myeloid (Spi1, PU.1) transcription factors. Data derived from comparison of two paired replicates from independent experiments in the Haemosphere database. (C) Frequency of transcript counts for Il1rl1 (encoding ST2) among indicated progenitor populations. RETIC, reticulocyte. Bars represent mean and SEM of two biological replicates from the Haemosphere database. (D) Representative images of flow cytometric expression of the ST2 receptor on indicated progenitor populations in BM of WT and ST2−/− (ST2 KO) mice. ST2−/− mice were on C57BL/6 background; WT mice were bred under the same conditions. HSCs: Lineageneg, Sca1+, cKit+, CD150+, CD48−; MPPs: LSK, CD150−, CD48+; pre-MEG-E, premegakaryocyte-erythroid progenitor (Lineageneg, Sca1−, cKit+, CD41−, CD16/32−, CD105−, CD150+). Representative of two independent experiments.