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. 2020 Sep 8;15(9):e0238572. doi: 10.1371/journal.pone.0238572

Fig 4. Protein expression levels of ERK and pERK in human and mouse rhabdomyosarcoma cell lines after treatment with Latrunculin A.

Fig 4

Human RD sarcoma cells were exposed to 250nM latrunculin A, human Rh30 RMS cells to 100nM latrunculin A and mouse RMS sarcoma cells to 100nM latrunculin A. Different concentrations were chosen due to differences in latrunculin A sensitivity between sarcoma cell lines. (A) Evaluation of ERK1/2 (42/ 44kDa), p-ERK1/2 (22/ 44 kDa) and GAPDH (37 kDa) by Western blotting demonstrated reduced ERK1/2 phosphorylation in latrunculin A-treated sarcoma cells. (B) The F-actin cytoskeleton was evaluated using phalloidin staining. Actin organization was profoundly disrupted in latrunculin A-treated (top right panel)) vs. control (top left panel) RD cells, latrunculin A-treated (middle right panel) vs. control (middle left panel) Rh30 cells and latrunculin A-treated (bottom right panel) vs. control (bottom left panel) mouse RMS cells. Please also see S1 Raw images.