Table 1. Proliferation-relevant sarcoma genes.

Transcriptional profiling of genetically engineered, KRAS-induced mouse rhabdomyosarcomas (RMS) and non-myogenic sarcomas (NMS) identified 141 sarcoma-relevant genes; their function was evaluated using a customized shRBA screen. Sixteen of the 141 sarcoma-relevant genes scored as „hits”in RMS and/ or NMS (p<0,01, q>0,05, at least 3 of 5 shRNAs with FDR< 30%) and are listed here. These 16 hits include 5 potentially druggable targets, i.e. Asns, Cenpe, Crebl2, HAs2 and Ube2c.

			Kras; p16p19null RMS				Kras; p16p19null NMS
Symbol	Cell function	Chemical modulation	Mean % of ctrl	Level	p-value	q value	Mean % of ctrl	Level	p-value	q value
Asns	Asparagine synthesis	AS5, Asparaginase, Mupirocin	30,16	2	< .0001	0,0058	6,6934	1	< .0001	0,0072
Rbbp8	Cell cycle		56,06	3	0,0022	0,0160	30,7323	2	0,0012	0,0288
Rad54l	DNA repair		57,56	3	0,0031	0,0180	40,5093	3	0,0076	0,0421
Ube2c	Cell cycle	Bortezomib	43,56	3	< .0001	0,0058	38,6873	-	0,0054	0,0389
Cenpe	Chromosome stability	UA62784, GSK923295A	72,49	-	0,0511	0,0689	39,9054	3	0,0067	0,0402
Has2	Extracellular matrix	Latrunculin A	61,76	-	0,0075	0,0335	41,5132	3	0,0088	0,0453
Creb3l2	Transcriptional regulation	Sorafenib	62,69	2	0,0090	0,0326	60,2497	-	0,1230	0,1736
Basp1	Transcriptional regulation		45,37	3	0,0002	0,0039	50,8361	-	0,0371	0,0989
Lrrfip1	Transcriptional regulation		54,80	3	0,0017	0,0164	54,4497	-	0,0606	0,1322
Lasp1	Cytoskeletal organization		60,37	3	0,0056	0,0271	58,6252	3	0,1019	0,1706
Pold3	DNA repair		62,96	3	0,0095	0,0290	31,9654	-	0,0015	0,0216
Efhd2	Apoptosis		53,27	-	0,0034	0,0179	46,8886	2	0,0007	0,0252
Myo9b	Cytoskeletal organization		67,41	-	0,0445	0,0737	33,5929	2	0,0021	0,0252
Runx1	Transcriptional regulation		64,64	-	0,0455	0,0713	30,6930	3	0,0012	0,0288
Shcbp1	Cell proliferation		71,42	-	0,1118	0,1099	37,7316	3	0,0045	0,0405
Egr2	Transcriptional regulation		74,44	-	0,0689	0,0850	42,9416	3	0,0048	0,0384