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. 2020 Aug 26;16(8):e1008745. doi: 10.1371/journal.pgen.1008745

Fig 5. Gnp1 dependent serine uptake is essential to maintain sphingolipid homeostasis.

Fig 5

(a) Serial dilutions of WT, gnp1Δ cells, agp1Δ cells and gnp1Δagp1Δ cells on synthetic medium. Control plates (upper left), plates without serine (upper right), plates containing 1 μM myriocin (lower left) and plates containing 1 μM myriocin with no serine available (lower right) are displayed. (b) Serial dilutions of WT, gnp1Δ cells, agp1Δ cells and gnp1Δagp1Δ cells on YPD medium. Control plates (left), plates containing 0.5 μM myriocin (middle) and plates containing 0.5 μM myriocin and 25 μM phytosphingosine (PHS) (right) are displayed. (c) Serial dilutions of prototroph WT, gnp1Δ cells, agp1Δ cells and gnp1Δagp1Δ cells on plates with synthetic medium without amino acids (upper row) and plates without amino acids (-AAs) with serine (lower row), each with 0.5 μM (middle) and 1.5 μM myriocin (right). (d) Tetrad analysis of orm1Δorm2Δ cells (dark blue and yellow, respectively) crossed with gnp1Δ (red) cells and followed by the deletion of AGP1 (green). (e) Quantification of tetrad analyzis shown in (d). Relative colony sizes of 35 tetrades of diploid orm1Δorm2Δgnp1Δagp1Δ cells shown as fold change from WT tetrades. Error bars represent standard deviations. *, P-value <0.05, calculated from t-test.