Table 2. Distributions of open durations for mutant α7 nAChRs.
| Calcium free | 1.8 mM Ca2+ | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean open, s | Weight | Mean open, s | Weight | |||||||||
| α7 E158A | 1.0 × 10−5 ± 1.1 × 10−6 | 8.2 × 10−5 ± 7.3 × 10−6 | 0.74 ± 0.02 | 0.26 ± 0.02 | 9.9 × 10−6 ± 1.1 × 10−6 | 2 × 10−5 ± 7.3 × 10−6 | 3.3 × 10−4 ± 4.5 × 10−6 | 0.54 ± 0.05 | 0.33 ± 0.06 | 0.12 ± 0.03 | ||
| n = 4 | n = 4 | |||||||||||
| α7 E185Q | 6.8 × 10−6 ± 2.3 × 10−5 | 5.0 × 10−5 ± 2.3 × 10−6 | 2.4 × 10−4 ± 5.3 × 10−5 | 0.40 ± 0.02 | 0.37 ± 0.02 | 0.23 ± 0.03 | 9.4 × 10−6 ± 2.0 × 10−5 | 6.5 × 10−5 ± 2.7 × 10−6 | 2.8 × 10−4 ± 3.8 × 10−6 | 0.50 ± 0.09 | 0.39 ± 0.05 | 0.10 ± 0.06 |
| n = 4 | n = 4 | |||||||||||
| α7 D160S | 1.0 × 10−5 ± 1.0 × 10−6 | 1.1 × 10−4 ± 1.0 × 10−5 | 0.69 ± 0.03 | 0.30 ± 0.03 | 1.0 × 10−5 ± 1.6 × 10−6 | 3.3 × 10−4 ± 1.7 × 10−5 | 0.71 ± 0.03 | 0.28 ± 0.03 | ||||
| n = 4 | n = 4 | |||||||||||
Time constants and relative weights of exponential components were obtained by fitting open duration histograms for n experiments. Duration values are mean open ± SD.