Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Efnb2flox | Grunwald et al., 2004 | Efnb2tm4Kln; RRDI: MGI:2182626 | |
| Genetic reagent (Mus musculus) | Ephb4flox | Martin-Almedina et al., 2016 | ||
| Genetic reagent (Mus musculus) | Pdgfb-iCreERT2iresGFP | Claxton et al., 2008 | Tg(Pdgfb-icre/ERT2)1Frut; RRDI: MGI:3793852 | |
| Genetic reagent (Mus musculus) | R26-mTmG | Muzumdar et al., 2007 | Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo; RRDI: MGI:3716464 | |
| Genetic reagent (Mus musculus) | Prox1-CreERT2 | Bazigou et al., 2011 | Tg(Prox1-cre/ERT2)1Tmak; RRDI: MGI:5617984 | |
| Genetic reagent (Mus musculus) | Efnb2GFP | Davy and Soriano, 2007 | Efnb2tm2Sor; RRDI: MGI:3526818 | |
| Genetic reagent (Mus musculus) | Cldn11-CreERT2 | This paper | H Ortsäter and T Mäkinen, manuscript in preparation. | |
| Genetic reagent (Mus musculus) | Cldn5flox | This paper | Cldn5tm1a(EUCOMM)Wtsi; MGI:5473167 | Obtained from The European Conditional Mouse Mutagenesis Program (EUCOMM). |
| Cell line (Homo sapiens) | Dermal lymphatic endothelial cell (normal, juvenile, male) | PromoCell | Cat# C12216 | Primary cell line isolated from foreskin, tested negative for mycoplasma contamination |
| Antibody | anti-EphrinB2 (Human single chain variable fragment) | Abéngozar et al., 2012 | B11 | (80 μg/ml) |
| Antibody | anti-human IgG (Goat polyclonal) | Jackson ImmunoResearch | Cat# 109-005-098 | (5 μg/ml) |
| Antibody | anti-human/mouse/rat EphrinB2 (Goat polyclonal) | R and D Systems | Cat# AF496 | (0.5 μg/ml) |
| Antibody | anti-human EphB4 (Goat polyclonal) | R and D Systems | Cat# AF3038 | (0.5 μg/ml) |
| Antibody | anti-human VE-cadherin (Goat polyclonal) | Santa Cruz Biotechnology | C19, Cat# sc-6458 | (IF: 0.2 μg/ml; WB 2 μg/ml) |
| Antibody | anti-human/mouse CLDN5 (Rabbit polyclonal) | Invitrogen | Cat# 34–1600 | (0.5 μg/ml) |
| Antibody | anti-phosphotyrosine 4G10 (Mouse monoclonal) | Merck | Cat# 05–321 | (0.5 μg/ml) |
| Antibody | anti-β-actin (Rabbit polyclonal) | Cell Signalling Technologies | Cat# 4967 | (0.1 μg/ml) |
| Antibody | anti-mouse Podoplanin (Syrian hamster monoclonal) | eBioscience | eBio8.1.1, PE; Cat# 12-5381-82; RRDI:AB_1907439 | (2 µg/ml) |
| Antibody | anti-mouse CD31 (Rat monoclonal) |
eBioscience | 390, PE-Cy7, Cat# A14715; RRDI:AB_2534231 | (0.7 µg/ml) |
| Antibody | anti-mouse CD45 (Rat monoclonal) |
eBioscience | 30-F11, PerCP-Cyanine5.5; Cat# 5-0451-82, RRDI:AB_1107002
or eFluor450; Cat# 48-0451-82; RRDI:AB_1518806 |
(4 µg/ml) |
| Antibody | anti-mouse CD11b (Rat monoclonal) | eBioscience | M1/70, PerCP-Cyanine5.5; Cat# 45-0112-82; RRDI:AB_953558 or eFluor450; Cat# 48-0112-82; RRDI:AB_1582236 | (4 µg/ml) |
| Antibody | anti-TER-119 (Rat monoclonal) | eBioscience | TER-119, eFluor450; Cat# 48-5921-82; RRDI:AB_1518808 | (4 µg/ml) |
| Antibody | anti-Ki-67 (Rat monoclonal) | eBioscience | SolA15, eFluor 660; Cat# 50-5698-82; RRDI:AB_2574235 | (1:100) |
| Antibody | anti-human VE-cadherin (Mouse monoclonal) | Santa Cruz Biotechnology | F-8, Cat# sc-9989 | (2 μg/ml) |
| Antibody | anti-mouse LYVE1 (Rat monoclonal) | R and D Systems | Cat# MAB2125 | (1 μg/ml) |
| Antibody | anti-GFP (Rabbit polyclonal) | Abcam | Cat# ab290 | (1 μg/ml) |
| Antibody | anti-mouse FoxC2 (Sheep polyclonal) | R and D Systems | Cat# AF6989 | (2 μg/ml) |
| Sequence-based reagent | TaqMan probe: Gapdh | ThermoFisher Scientific | Cat# Mm_03302249_g1 | |
| Sequence-based reagent | TaqMan probe: Efnb2 | ThermoFisher Scientific | Cat# Mm00438670_m1 | |
| Sequence-based reagent | siRNA: human CLDN5 | Dharmacon | Cat# D-011409-03-0010 | |
| Sequence-based reagent | siRNA: human CDH5 | Dharmacon | Cat# D-003641-03-0010 | |
| Sequence-based reagent | siRNA: human EFNB2 | Dharmacon | Cat# L-003659-00-0005 | |
| Sequence-based reagent | siRNA: negative control, human | Qiagen | Cat# 1027281 | |
| Peptide, recombinant protein | Human EphrinB2-Fc | R and D Systems | Cat# 7397-EB | (0.5 μg/ml: activation assay, 3 μg: IP) |
| Chemical compound, drug | ROCK inhibitor (Y-27632) | Sigma | Cat# SCM075 | (10 µM) |
| Commercial assay or kit | Rac1-GTP pulldown assay | Cytoskeleton Inc | Cat# BK030 |