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. 2020 Aug 26;8:827. doi: 10.3389/fcell.2020.00827

FIGURE 4.

FIGURE 4

circ-0016068 enhances BMI-1 expression by binding to the 3’-UTR of miR-330-3p. (A) Representative images of the subcellular localization of circ-001068 by RNA-FISH in prostate cancer cells. (B) Evaluating the correlation of circ-0016068 and BMI-1 expression in prostate cancer cells by Pearson’s correlation coefficient. (C) Evaluating the correlation of circ-0016068 and BMI-1 expression in prostate cancer tissues by Pearson’s correlation coefficient. (D) The effect of circ-0016068 knockdown or overexpression on the expression of BMI-1 in prostate cancer cells by western blotting. (E) Bioinformatics analysis with PITA, microT, TargetScan, and circinteractome. (F) The level of miR-330-3p in prostate cancer tissues. (G) The level of miR-330-3p in prostate cancer cells. (H) The level of BMI-1 in prostate cancer cells. (I) Dual-luciferase reporter assay and the binding sites. (J) The effect of miR-330-3p on the expression of BMI-1 in prostate cancer cells by western blotting. (K) The effect of miR-330-3p inhibitor and shRNA-circ-0016068 on the expression of BMI-1 in prostate cancer cells by western blotting. (L) Overexpressing a fragment of circ-0016068 that contains the MRE of miR-330-3p could reversed the inhibition of BMI-1 expression induced by miR-330-3p overexpression. Data represent the mean ± SD of 3 independent experiments. **p < 0.01; ***p < 0.001.