Fig. 2.

Evolutionary tree of the family GH126. The tree consists of 117 unique non-redundant sequences of the family GH126 (all from Firmicutes) and 17 additional potential family members originating outside the phylum Firmicutes. The tree is based on the alignment of complete sequences (for details, see Fig. S1). The two large evolutionary groups identified previously (Kerenyiova and Janecek 2020) represented by the CPF_2247 amylolytic enzyme from C. perfringens (48 members; red colour) and the PssZ protein from L. monocytogenes (69 members; blue colour) are completed by additional out-of-Firmicutes sequences coloured green. Each protein is labelled by the name of the organism and the GenBank accession number. Four proteins containing the N-terminal extensions that were cut for making the alignment are marked by an asterisk; the length of the extension being indicated in parentheses. With regard to bootstrap values (not shown to preserve the clarity), they were ≥ 50% for more than 83% of interior branches