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. 2020 Aug 6;13(4):359–367. doi: 10.1007/s12195-020-00639-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Neutrophils undergo spontaneous NETosis in the presence of tumor cells. (a) cfDNA plasma DNA level of normal donors (ND) and cancer patients (PAT). (b) Confocal images of neutrophils that underwent NETosis in conditioned media. (CTL)—control group, COLO-205 and SW-620 are conditioned media collected after 48 h from these indicated cell lines. (c) NETosis level of neutrophils after culturing in conditioned media for 24 h. (d, e) Extracellular tumor-derived IL-8 levels in conditioned media. Reparixin concentration was 20 μg/mL.