Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 6;13(4):359–367. doi: 10.1007/s12195-020-00639-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Engineered neutrophils express supercharged eGFP-TRAIL on NETs during NETosis. (a) Schematic of the insertion site, AAVS1, on chromosome 19. Cartoon representation of eGFP-TRAIL chimeric protein. Surface charge of eGFP ranging from − 4 to + 36 (Red—negative charge, Blue—positive charge. (b) DNA sequencing result of genomic DNA isolated from cells positive for eGFP-TRAIL. (c) relative mRNA level of endogenous (enTRAIL) and exogenous (exTRAIL) TRAIL levels. (d) Flow cytometry of cells expressing eGFP-TRAIL 24 h after nucleofection. (e) Confocal images of NETs decorated with eGFP-TRAIL (Blue—DAPI stain, Green—eGFP-TRAIL). (f) Immuno-gold SEM images of neutrophils expressing eGFP-decorated NETs (White arrows—eGFP-TRAIL).