Table 1.

Primers Used in the Study.

Primer name (purpose)	Sequence
hNLRP2 (Add new EcoRI site and frame-correction)	F: 5′- CCACGTGGGACAAGAATTCATGGTGTCTTCGGC-3′
	R: 5′- GCCGAAGACACCATGAATTCTTGTCCCACGTGG-3′
hNLRP2-EcoRI (Insert EcoRI at the end PYD of pcDNA3FLAG-hNLRP2)	F: 5′-GCTTTGAAATCCTTGAATTCAAGGAAGCCTCTATC-3′
	R:5′-GATAGAGGCTTCCTTGAATTCAAGGATTTCAAAGC-3′
hNLRP332 (Amplify LRRs; 7901062 from NLRP2 with BamHI and XhoI sites to swap with LRRs of NLRP3;718-1034)	F: 5′-GGATCCTTGGTGTCTTGTTCCGCTAC-3′
	R: 5′-CTCGAGTCAGATCATGAAGTCATGAGAAG-3′
hNLRP3 1-797 (introduce a stop codon at aa 797)	F: 5′-CAGCAGCAACCAGAAGTAGGTGGAGCTGGACCTG-3′
	R: 5′-CAGGTCCAGCTCCACCTACTTCTGGTTGCTGCTG-3′
hNLRP3 1-532 (introduce a stop codon at aa 532)	F: 5′-CTTTGCCGCCATGTACTACTAGCTGGAAGAGGAAAAGG-3′
	R: 5′-CCTTTTCCTCTTCCAGCTAGTAGTACATGGCGGCAAGG-3′
hNLRP3 1-432 (introduce a stop codon at aa 432)	F: 5′-CAAGAGCCTTGCCTAGA CATCCAAGACCACCACCGCGG TGTACG-3′
	R: 5′-CTGGACTGAAACAGCAGATGGAGAGTGGCAAGAGCCTTG CCTAGACATC-3′
hNLRP3 1-134 (introduce a stop codon at aa 134)	F: 5′-TCCATATGTAAAATGAAGAAATGATTACCGTAAGAAGTAC-3′
	R: 5′-GTACTTCTTACGGTAATCATTTCTTCATTTTACATATGGA-3′
hNLRP233 (Amplify hNLRP2 PYD with KpnI site; allows NLRP2-PYD insertion in NLRP3ΔPYD)	F: 5′-GGTACCTGGAGCAGCTCAGCCAGG-3′ R: 5′-GGTACCTTTATTAAAGGATTTCAAAGCTGC-3′
hNLRP133 (Amplify hNLRP1 PYD with KpnI site; allows NLRP1-PYD insertion in NLRP3ΔPYD)	F: 5′-GGTACCTGGAGTTCCTGAAGAAGGAG-3′ R: 5′-CATTCCCCTACAGCCCAAGGTACC-3′
hNLRP3C8S/A (Mutate C8 to S, A, or G)	F: 5′-AAAATGGCAAGCACCCGCKSCAAGCTGGCCAGGTACCTG-3′
	R: 5′-CAGGTACCTGGCCAGCTTGSMGCGGGTGCTTGCCATTTT-3′
hNLRP3C38S/A (Mutate C8 to S, A, or G)	F: 5′-ATCCTCCCCAGAAGGGCKSCATCCCCCTCCCGAGGGG-3′
	R: 5′-CCCCTCGGGAGGGGGATGSMGCCCTTCTGGGGAGGAT-3′
hNLRP3C108S/A (Mutate C8 to S, A, or G)	F: 5′-GAATCCCACTGTGATAKSCCAGGAAGACAGCATTG-3′
	R: 5′-CAATGCTGTCTTGGSMTATCACAGTGGCATTC-3′
pCX4-Puro-NLRP3 (Retroviral cloning of NLRP3 and mutants)	F:5′-CCATCCTCTAGACTGCCGGATCCATGGACTACAAGGACGATGACG-3′
	R: CCGCACGCGTCGGTCCGGAATTCCTACCAAGAAGGCTCAAAGACG-3′