Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 8;11:4480. doi: 10.1038/s41467-020-18153-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Distribution of mCherry-tagged CCT isoforms in MEF. (a) Regular medium. (b) Starvation for 1 h. Bar, 10 μm. b Distribution of CCTβ3-mCherry (magenta) and Atg proteins (green) in MEFs starved for 1 h. Bar, 5 μm. The colocalization ratios of Atg proteins and CCTβ3-mCherry in the original picture (gray bar) and in the picture after 90° clockwise rotation of the green image (white bar) are shown. Pooled data obtained in three independent experiments (n = 60); *p = 0.0319, ****p < 0.0001 (two-tailed Mann–Whitney test). c PC synthetic activity. ³H-choline was added to medium 30 min before harvest. (a) MEFs with or without CCT overexpression cultured in regular medium or starvation medium for 1 h. (b) The result in starvation medium is normalized by the expression level of CCT isoforms, estimated by the CCT-mCherry band intensity in western blotting (c). A representative result of three independent experiments. d The effect of CCT overexpression on GFP-DFCP1 in MEFs starved for 1 h. Bar, 10 μm. (i) The punctum size. The number of puncta counted: 578 (WT), 486 (CCTα), 481 (CCTβ2), and 2320 (CCTβ3). (ii) The total area in a cell. The number of cells counted: 90. Pooled data from three independent experiments. ****p < 0.0001 (two-tailed Mann–Whitney test). e The effect of CCTβ3 overexpression on the autophagic flux estimated by western blotting of LC3. MEFs were cultured for 1 h in starvation medium, with or without the addition of 20 μM chloroquine (CQ) for the last 30 min. A representative result of three independent experiments. In box plots, the center line indicates the median, box boundaries indicate the 25th and 75th percentiles, whiskers are Tukey-type, and the average is marked as +. Source data are provided as a Source data file.