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NMJ morphology in the ipsilateral hemi-diaphragm. No significant alterations were seen among groups and most of the NMJs were intact. Nicotinic acetylcholine receptors stained with α-bungarotoxin (in blue, A–D) and pre-synaptic terminals stained with SMI-312 and SV2 (in green, A’–D’). (A”–D”): merged images. (E) Quantification of intact, partially-denervated and completely-denervated NMJs at the ipsilateral hemi-diaphragm. Data is presented as median ± IQR (n = 3 per group).
