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. 2020 Sep 8;10:14787. doi: 10.1038/s41598-020-71640-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Colony spreading assay results for mecC-S. aureus with different agr functionalities on soft agar plates. A TSA soft agar plate (0.24%) was inoculated with 2 µl overnight culture of S. aureus. Examples shown here include isolate RKI5966 associated with a weak agr functionality, which was not able to spread on semisolid agar plates, while isolate IMT38119 (strong agr functionality) showed spreading. As controls we have employed the standard laboratory strains RN4220 (weak agr functionality) and USA300 (strong agr functionality).