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. 2020 Sep 8;11:4464. doi: 10.1038/s41467-020-18130-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic of the modular platform for the detection of SARS-CoV-2. Viral RNA is isolated using the Analytik Jena FeliX liquid handler with the Analytik Jena or Promega RNA extraction kits. Sample RNA is transferred and detection reactions are set up using the Labcyte Echo platform. These include qPCR, validated for the TaqPath (Thermo Fisher Scientific), Luna (NEB), and Fast Virus (Thermo Fisher Scientific) RT-qPCR master mix options, RT-LAMP nucleic acid detection, and the CRISPR-Cas13a diagnostic workflow. b Automated RNA extraction was developed using VLP dilutions of 10³ and 10⁴ copies/mL for the Analytik Jena and Promega RNA extraction kits. Efficiency of the extractions using both kits was analysed by RT-qPCR with the CDC N1 primer–probe set using the TaqPath master mix. c Dilutions of VLPs used for RT-qPCR in b were analysed using the CRISPR NAT to demonstrate the use of this workflow as an alternative diagnostic option. Error bars in b represent mean ± SE of n = 3 biologically independent samples with three technical replicates. Error bars in c represent mean ± SE of n = 3 independent amplification replicates and four technical replicates for CRISPR detection. Source data are available in the Source Data file.