Figure 2.

FCS measurements reveal impaired oligomerization dynamics for GFP-tagged Drp1 in vitro. (A–D) Autocorrelation curves for the various fluorescently labeled Drp1 constructs in the absence and presence of the indicated additives obtained using single-point FCS. In each case, the corresponding fluorescent label alone (mEGFP or small organic dye) was also measured. The recovered ‘D’ from the fitting of the data (solid lines) to one or two species are shown in Table 1. The data and fits were normalized to facilitate direct comparisons. Apoprotein refers to the indicated fluorescently labeled Drp1 construct in the absence of nucleotide. Autocorrelation plots were prepared using matplotlib v3.2.2 available at https://matplotlib.org/3.2.2/api/_as_gen/matplotlib.pyplot.boxplot.html.