Table 2.
Diffusion coefficients (D) and particle number concentrations (C) for cytosolic mEGFP alone and mEGFP-Drp1 overexpressed in live cells and measured using RICS.
| D, μm2/s | C, nM | n | |
|---|---|---|---|
| mEGFP alone (HeLa) | 22.4 ± 3.29 | 345 ± 207 | 11 |
| mEGFP-Drp1 (HeLa) | 1.54 ± 0.72 | 115 ± 95.4 | 13 |
| mEGFP alone (wt MEF) | 26.2 ± 1.49 | 331 ± 57.9 | 10 |
| mEGFP-Drp1 (wt MEF) | 2.18 ± 0.55 | 58.5 ± 33.6 | 12 |
| mEGFP alone (Drp1-null MEF) | 23.9 ± 3.57 | 375 ± 189 | 13 |
| mEGFP-Drp1 (Drp1-null MEF) | 2.45 ± 0.65 | 25.9 ± 7.77 | 11 |
n number of cells measured (one RICS measurement per cell).
Values reported are mean ± S.D.