Fig. 3.

Representation of a pooled CRISPR screen. Lenti- or retroviral particles are typically used for delivery as they can (i) be titrated to achieve a specific infection rate, (ii) will integrate into the genome of the infected cell, and (iii) infect many different cell types. The integration enables simple quantification of the gRNA representation in different cell populations by next-generation sequencing, and the subsequent identification of enriched or depleted gRNAs comparing different populations.