Figure 2.

Comparison of the Characteristics of T2D Models of WT, KL^+/−, and TgKL Mice

T2D models of WT, KL^+/−, and TgKL male mice were given a continuous HFD (n = 8/group). (A–I) SerumsKL levels (A), BW change (B), fasting blood glucose levels (C), fasting blood insulin levels (D), QUICKI (E), GTT (F), serum levels of inflammatory factors (TNF-α and IL1-β) (G), hepatic glycogen content (H), and hepatic 2-deoxyglucose (2-DG) uptake levels (I) were determined. (J) H&E staining, PAS staining, Masson staining, and IHC staining of sKL of liver sections from the three groups. Scale bars, 50 μm. (K) The protein levels of sKL, GCK, and PEPCK in livers of the three groups. (L–N) Protein levels of sKL (L), hepatic 2-DG uptake levels (M), and protein levels of GCK and PEPCK (N) were determined in HG-FFA-treated L02 cells with overexpression of sKL or not. For all statistical plots, the data are presented as the mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (F) ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; †p < 0.05, ††p < 0.01, †††p < 0.001; KL^+/− DM, TgKL DM compared with WT DM, respectively.