Figure 5.

The PI3K/AKT/mTORC1 Signaling Pathway Mediated the Regulation of sKL on PPARα Expression

WT DM, KL^+/− DM, and TgKL DM were fed with a HFD for 8 weeks (n = 8/group). (A and B) The levels of PI3K (PI3KP85α and PI3KP110α), p-AKT, AKT, p-mTOR, and mTOR (A) and phosphorylation levels of P70S6K and 4EBP1 (B) in livers of the three groups were subjected to western blot analysis. (C) Levels of AKT, mTOR, P70S6K, 4EBP1, and PPARα in HG-FFA-treated pLV-sKL L02 cells were tested. (D) Levels of p-AKT, p-mTOR, p-P70S6K, p-4EBP1, and PPARα in HG-FFA-treated pLV-sKL L02 cells treated with 740 Y-P or NV-5138 or not were determined. (E) Oil red O staining of HG-FFA-treated pLV-sKL L02 cells treated with 740 Y-P or NV-5138 or not were performed. Scale bar, 50 μm. For all statistical plots, the data are presented as the mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.