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Final Established HPLC Method: 370 mM NaCl Step Gradient Column Wash after Sample Injection and 440 mM NaCl Step Gradient Elution of LVs in 20 mM Tris-HCl (pH 7.5)
(A) HPLC chromatographic profile shows one single LV peak eluted at 6.24 min for SC-LV and 3 dpi samples. (B) A standard curve was generated using the SC-LV standard, with the linearity range of 3.13 × 108 to 1.0 × 1010 TP/mL. The intra-assay (repeatability) and inter-assay (day-to-day) variabilities are less than 5% RSD.
