Subject	Microbiology
Specific subject area	Applied Microbiology
Type of data	Table Figure 16S rDNA illumine sequences
How data were acquired	Amplicon metasequencing using Illumina HiSeq platform
Data format	Raw data in FASTQ format Analyzed
Parameters for data collection	Fecal samples were collected for microbial analysis from adolescents over 15 years old, who signed voluntary informed consent forms. The data collection was approved by the Ethics Committee of Scientific centre for Family Health and Human Reproduction Problems (protocol N6 from 21 December 2015). Adolescents were grouped according to body mass index into three groups: normal body mass (23 persons), obesity (BMI ≥ 95 percentile) (20), and obesity with irritable bowel syndrome (IBS) (10).
Description of data collection	Total DNA was extracted from stool samples, the V3-V4 variable regions of 16S rDNA were amplified, and paired-end sequenced according to Illumina protocol using Illumine platform.
Data source location	Institution: Scientific centre for Family Health and Human Reproduction Problems City: Irkutsk Country: Russia
Data accessibility	Raw data were deposited to NCBI Repository name: SRA Data identification number: BioProject PRJNA604466, BioSamples from SAMN13972309 to SAMN13972361. Direct URL to data: https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA604466
Related research article	Belkova N.L., Nemchenko U.M., Pogodina A.V., Feranchuk S.I., Romanitsa A.I., Novikova E.A., Rychkova L.V. Composition and Structure of Gut Microbiome in Adolescents with Obesity and Different Breastfeeding Duration. // Bulletin of Experimental Biology and Medicine. 2019. V. 167, No. 6. P. 759–762. Translated from Byulleten’ Eksperimental'noi Biologii i Meditsiny, Vol. 167, No. 6, pp. 717–721. DOI 10.1007/s10517–019–04617–7