Figure 3.

ONCR-159 Incorporates Multiple Modifications Including Next-Generation miR-T Cassettes That Are Efficiently Attenuated by Multiple miRNAs

(A) Depicts the vector maps of the predecessors leading to ONCR-159. ONCR-159 contains 4 miR-T cassettes and the UL37 mutations. (B) Schematic representation of the overall miRNA attenuation strategy including tissue protected, miRNAs selected from each tissue group, and the HSV-1 gene of interest where the miR-T has been inserted. Green represents the CNS, orange smooth, and cardiac/skeletal muscle, and blue soft tissue (e.g., liver and pancreas) protection. (C) ONCR-159 viral replication in miRNA-mimic pretreated A253 cells is shown as a ratio of the negative control mimic (PFU/neg). The miRNA mimics is labeled on the x-axis. Statistical significance was calculated using Bonferroni-Dunn Student’s t-test, ∗p = 0.02, ∗∗∗∗p < 0.00001. CNS, central nervous system; PFU, plaque forming units; Neg, negative.