Figure 2.

AAV DNA Distribution in the Heart at Different Time Points after i.v. Injection

AAV DNA distribution in the heart at 2 and 24 h, 5 and 8 days, and 2, 4, 12, and 24 weeks after i.v. injection. Cyan arrowhead points to sarcolemma; red arrow points to cytoplasmic AAV DNA; yellow arrow points to nuclei without AAV DNA; blue arrow points to nuclei with AAV DNA. Bottom rows are the high-magnification images of the green boxed areas in the corresponding top row images. Scale bars, 50 μm (for both ×20 and ×60 original magnification).