Figure 8.

AAV DNA Distribution in the TA Muscle at Different Time Points after i.m. Injection

At 2 and 24 h after i.m. injection, a considerable amount of the AAV DNA was seen along the muscle membrane, with occasional cytoplasmic and nuclear localization at 24 h after injection. From 5 days to four weeks after i.m. injection, most of the AAV DNA resided in the nuclei (blue arrow), with isolated cytoplasmic distribution (red arrow). From 12 to 24 weeks, no viral DNA was seen intercellularly or intracellularly. Arrowhead points to AAV DNA at the membrane; red arrow points to AAV DNA in the cytoplasm; yellow arrow points to nuclei without AAV DNA; blue arrow points to nuclei with AAV DNA. Scale bars, 50 μm (for both ×20 and ×60 original magnification).