Table 3.
Name | Strain | Propagated in | Medium* | Procured from |
---|---|---|---|---|
HCoV | 229E | Huh-7, 33 °C | DMEM + 5% FBS, 2 mM Glutamine, non-essential amino acids, Pen/strep, HEPES (Biochrom, Germany) | Prof. Volker Thiel, University of Bern, Switzerland [24, 25] |
MERS-CoV | EMC | Vero, 37 °C | DMEM + 2% FBS, 2 mM Glutamine, non-essential amino acids, Pen/strep, HEPES (Biochrom, Germany) | |
SARS-CoV | Frankfurt-1 | |||
SARS-CoV-2 | BetaCoV/France/IDF0372/2020 | Vero E6, 37 °C | Institute Pasteur, Paris, France via EVAg, European Virus Archive. | |
Mouse parvovirus | MVM Prototype, ATCC-1346 | A9, 37 °C | The National Collection of Pathogenic Viruses, UK | |
Yellow Fever virus | 17D, NCPV-0507 | Vero, 37 °C | ||
Vaccinia virus | Elstree (Lister Vaccine), ATCC-VR-1549 |
All viruses were cultured without CO2 in non-vented flasks, 24 well-, or 96 well-plates covered with sealing foil (Biorad, microseal B-film, MSB 1001) for the duration of experiments
aDulbecco’s Modified Eagle Medium (DMEM), Minimum Essential Medium (MEM), Fetal Bovine Serum (FBS), Penicillin/Streptomycin (Pen/Strep, 100 U/mL)