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. 2020 Sep 9;17:136. doi: 10.1186/s12985-020-01401-2

Table 3.

Overview of viruses used in the current study

Name Strain Propagated in Medium* Procured from
HCoV 229E Huh-7, 33 °C DMEM + 5% FBS, 2 mM Glutamine, non-essential amino acids, Pen/strep, HEPES (Biochrom, Germany) Prof. Volker Thiel, University of Bern, Switzerland [24, 25]
MERS-CoV EMC Vero, 37 °C DMEM + 2% FBS, 2 mM Glutamine, non-essential amino acids, Pen/strep, HEPES (Biochrom, Germany)
SARS-CoV Frankfurt-1
SARS-CoV-2 BetaCoV/France/IDF0372/2020 Vero E6, 37 °C Institute Pasteur, Paris, France via EVAg, European Virus Archive.
Mouse parvovirus MVM Prototype, ATCC-1346 A9, 37 °C The National Collection of Pathogenic Viruses, UK
Yellow Fever virus 17D, NCPV-0507 Vero, 37 °C
Vaccinia virus Elstree (Lister Vaccine), ATCC-VR-1549

All viruses were cultured without CO2 in non-vented flasks, 24 well-, or 96 well-plates covered with sealing foil (Biorad, microseal B-film, MSB 1001) for the duration of experiments

aDulbecco’s Modified Eagle Medium (DMEM), Minimum Essential Medium (MEM), Fetal Bovine Serum (FBS), Penicillin/Streptomycin (Pen/Strep, 100 U/mL)