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. 2020 Aug 25;32(8):108054. doi: 10.1016/j.celrep.2020.108054

Figure 1.

Figure 1

Juvenile Zebrafish Can Perform Conditioned Place Avoidance Learning with Increased Performance across Development

(A) The top row shows a schematic representation of the protocol. B, baseline; C1, conditioning 1; T1, test 1; C2, conditioning 2; T2, test 2. Consecutive rows show the heatmaps depicting the average density of zebrafish position for each experimental group: 1-week-old (n = 36), 2-week-old (n = 24), 3-week-old (n = 24), and 4-week-old (n = 18) zebrafish.

(B) Relative time spent in the conditioned zone (CZ).

(C) Relative distance of swimming in the CZ.

(D) Average distance of the animals away from the CZ. Dashed line indicates the midline.

(E) Average swim velocity.

(B–E) Line color and style indicate different age groups. Data displayed in 2-min bins; mean ± SEM; #, indicate statistical comparisons detailed in Figures S1E and S1F.

(F) Histograms of CPA learning indices during the two test sessions. The darker colors represent the animals classified as learners. The percentage of learners is in the pie chart. See also Figures S1 and S2.