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. 2020 Sep 8;10:14772. doi: 10.1038/s41598-020-71256-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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IL-36γ mRNA expression and protein levels in HaCaT cells stimulated with LMW HA and Poly(I:C). Confluent HaCaT cells were stimulated with either media alone (Media), LPS, LWM HA (HA), LPS plus TAK-242 (LPS + TAK) or LMW HA plus TAK-242 (HA + TAK). IL-36γ mRNA expression and protein levels were quantified by real-time RT-PCR and ELISA. Each histogram shows the mean ± SEM values (n = 6; *p < 0.05; **p < 0.01).