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. 2020 Aug 20;23(9):101480. doi: 10.1016/j.isci.2020.101480

Figure 1.

Figure 1

WM266.4 Human Melanoma Cells form Aggressive and Indolent Brain Metastatic Lesions

(A and B) Representative images of WM266.4-Luc-EGFP brain metastasis at the indicated days after intra-cardiac injection. Sagittal brain sections are stained for Ki67 and whole slices (A) and enlarged views (B) are shown. Colored arrowheads indicate four stages (SC, MC, MM, and EM) of brain metastasis progression. Green: WM266.4 cells, Blue: DAPI, Red: Ki67. Scale bar, 2.5 mm (A), 100 μm (B).

(C and D) The number of lesions per sagittal brain section (C) and the proportion of SC and MC that contain at least one Ki67-positive cell (D) at the indicated days; 30 brain slices from 8 mice (day10), 32 brain slices from 8 mice (day 20), and 28 brain slices from 8 mice (day 30) were used for quantification.

(E and F) Size of lesions (number of cells) and Ki67 index in MM and EM at day 20 (E) and day 30 (F). Av., average of Ki67-index (n = 117 [MM at day 20], 64 [EM at day 20], 123 [MM at day 30], and 82 [EM at day 30]).

Data are mean ± SEM (C and D).