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. 2020 Aug 20;23(9):101480. doi: 10.1016/j.isci.2020.101480

Figure 2.

Figure 2

Non-proliferating WM266.4 Cells Exhibit the Gene Expression Signature of DNMT1 Inhibition

(A) Fragments per kilobase of exon per million reads mapped (FPKM) of MKI67 in the 61 single cell-derived cDNA libraries, separated into Non-proliferating (23 cells, FPKM <1) and Proliferating (38 cells, FPKM >1) group.

(B and C) FPKM of cell proliferation-related genes (TK1, MCM7, TOP2A and PCNA) in Non-proliferating (NP) and Proliferating (P) group in the single cell-derived cDNA libraries.

(D) GSEA results with the indicated gene sets between Non-proliferating (NP) and Proliferating (P) groups.

(E) Representative images of WM266.4 brain metastasis, stained for DNMT1. Green: WM266.4 cells, Blue: DAPI, Red: DNMT1. Scale bar, 100 μm.

(F) Size of lesions (number of cells) and DNMT1-positive ratio in SC, MC, MM, and EM at day 30. Av., average of DNMT1-positive ratio (n = 37 [SC], 32 [MC], 39 [MM], and 21 [EM]). Data are mean (B and C).