Figure 4.

Validation of the Estimated Flux with Experimental Data Using ¹⁴C-Glucose

Error bars indicate 90% confidence intervals for the measurements and the estimates. The 90% confidence intervals for the measurements were calculated by the means ± z × the standard errors, where z is the Z score for 90% confidence intervals (1.6449). Estimated amounts were obtained by integrating sum of products of corresponding fluxes and mass isotopomer fractions of precursor metabolites (see Transparent Methods).