Figure 5.

Key Regulatory Events by Enzyme Phosphorylation, Allosteric Effectors, Substrates, and Products

(A) Overview of the method for quantifying the contribution of regulatory events. Delta (Δ) indicates difference between the Ins and Ctrl conditions.

(B) Clustering of reactions by key regulatory events. Hierarchical clustering was performed on regulation coefficients of ρP, ρA, ρS, and ρU at 1, 5, 10, 20, and 60 min using Euclidean distance as a metric with the Ward method. The data matrix used for the clustering contains 44 rows for reactions and 4,020 columns (5 time points × 4 ρ × 201 optimal and sampled parameters) (Table S4), and the mean of ρ among optimal and sampled parameters is shown as color in the heatmap. See Figures S4A and S8, Tables S3 and S4, and Data S1.

(C) The metabolic network shown with each reaction colored according to its cluster classification.