Figure 6.

Determination of Key Regulatory Molecules

(A) Time-averaged regulation coefficients for reactions with 90% confidence intervals of ρ_P or ρ_A > zero. Error bars indicate the 90% confidence intervals. The confidence intervals were calculated from 200 sets of sampled parameters (see Transparent Methods).

(B) Effect of key regulatory molecules on kinetic flux models. The dots indicate estimated flux changes over time by metabolic flux analysis under non-steady-state conditions ($\mathit{v}$), and the lines indicate kinetic fluxes (${\mathit{v}}^{kinetic}$) for the indicated metabolic enzymes. Kinetic fluxes with (right) or without (left) the indicated key regulatory molecules. Shown are the regulatory molecules that produced the smallest AIC when added to the model calculated with substrates and products. R2 and AIC values are indicated. A lower or more negative AIC value represents better fit. Note that kinetic fluxes through Glut4 without regulatory molecules in the Ins and Ctrl conditions are the same and the red and blue lines are overlapped. See also Figure S5A, Tables S3 and S4, and Data S1.