Fig. 5.

Dysfunctions induced by high glucose in hRMECs were regulated by circ_0002570-miR-1243-AMOT axis. hRMECs were treated with high glucose (HG) for 48 h after co-transfection with negative control siRNAs and negative control of miRNA inhibitor (si-NC + NC-inhibitor), a specific hsa_circ_0002570 siRNAs and negative control of miRNA inhibitor (si-circ_0002570-1 + NC-inhibitor) or a specific hsa_circ_0002570 siRNAs and miR-1243 inhibitor (si-circ_0002570-1 + miR-1243-inhibitor). a The RNA levels of miR-1243 and angiomotin in hRMECs were measured by qRT-PCR. n = 3. b The protein levels of angiomotin in hRMECs were determined by western blot. n = 6. c Cell viability was measured by CCK-8 assay. d Cell migration was assessed using transwell assay. n = 6. e Angiogenesis was evaluated by tube formation assay. n = 6. f The protein concentrations of VEGF in conditional medium of hRMECs were measured by ELISA. n = 6. g–j The protein levels of pro-inflammatory cytokines (TNF-α, IL-8, IL-6, and MCP-1) were evaluated by ELISA assay. n = 6. The data were represented as means ± SD. *P < 0.05; **P < 0.01