Figure 3.

Zebrafish tank with lid. (A) Each zebrafish tank is made of 5 mm transparent acrylic sheet, gluing with marine-safe acrylic glue. Each can hold 6 L water, making each tank could house a total of 30 zebrafish (5 fish/L). The outlet of the tank is constructed by drilling a hole on one side of the tank, and fix with a PVC fitting V-tank connector and an equal elbow. A mesh is installed onto the outlet, preventing fish from reaching the drainage line. (B) Each tank received continuous flow of water from the supply line. Excess water will then flow into the drainage line through the outlet. (C) The tank lid contains 2 openings used for feeding and as water inlet.