Figure 2. Timing of electrical pulse stimulation/contractions in myotubes in cell culture.

C2C12 myotubes were synchronized with dexamethasone and then subjected to electrical pulse stimulated for 1 h to induce twitch‐like contractions (lightning bolt). The timing was aligned based upon the temporal oscillation of Bmal1 mRNA content in the cells. Because C2C12 myotubes do not have rest/active phases, timing of contractions is presented as hours post‐synchronization (h p.s.) [Color figure can be viewed at wileyonlinelibrary.com]