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PCR primers for real-time PCR assay.
| Target strain | aPre/post primer | Primer sequence | Amplification length (bp) | References |
|---|---|---|---|---|
| Total bacteria | F | CGGCAACGACCGCAACCC | 130 | (35) |
| R | CCATTGTAGCACGTGTGTAGCC | |||
| Total fungi | F | GAGGAAGTAAAAGTCGTAACAAGGTTTC | 120 | (35) |
| R | CAAATTCACAAAGGGTAGGATGATT | |||
| Total protozoa | F | GCTTTCGWTGGTAGTGTATT | 223 | (34) |
| R | CTTGCCCTCYAATCGTWCT | |||
| Total methanogens | F | TTCGGTGGATCDCARAGRGC | 140 | (36) |
| R | GBARGTCGWAWCCGTAGAATCC |
F, forward; R, reverse.
