Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 26;11:1947. doi: 10.3389/fmicb.2020.01947

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Luscher, Simonin, Falconnet, Valot, Hocquet, Chanson, Resch, Köhler and van Delden.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Scheme of the infection and treatment schedule. 1 × 10³ bacterial cells are added at t = 0 h to a well seeded with 5 × 10⁵ Calu-3 cells. After 6 h incubation PAO1 has reached approximately 10⁶ CFU/well, at which time ciprofloxacin (CIP) or three different phages alone or in combination are added apically. Incubation is continued until 24 or 72 h, at which time point epithelial cell integrity (TER measurement), bacterial load (CFU) and phage titers (PFU) were determined.