FIGURE 4.

Measurement of trans-epithelial resistance (TER), CFU and PFU at 24 and 72 h p.i. of Calu-3 cells infected by P. aeruginosa PAO1 and treated by ciprofloxacin (CIP) or phages. (A) Single phage or CIP rescued PAO1-infected wild type Calu-3 cells at 24 h but not at 72 h p.i. (B) PAO1 viability is reduced at 24 h by phage or CIP but returns to almost untreated control levels after 72 h p.i. (C) phage replication increases during 72 h in wells containing PAO1 bacteria. Values represent mean and SEM (panel A), and median and range (panels B,C) of three independently performed experiments. Differences in TER between the conditions were assessed using a one-way ANOVA; **p < 0.01, ***p < 0.005.