FIGURE 6.

Cytotoxicity of bacteria surviving phage treatment. The cytotoxicity of PAO1-derivatives PT2426 (mucoid) and PT2527 (pyomelanin producer) were tested in absence and presence of phage cocktail/CIP treatment at 24 and 72 h p.i. on CTL (A) and cftr- cells (B). The bacterial load at the apical side of CTL (C) and cftr- cells (D) was determined by plate counting on LB-agar plates at 24 h and 72 h p.i. Values represent mean and SEM (panel A,B), and median and range (panels C,D) of three independently performed experiments. Differences in TER between the conditions were assessed using a two-tailed t-test; ^∗p < 0.05, ^∗∗p < 0.01.